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Wohlwend GmbH hpf 01 instrument
Hpf 01 Instrument, supplied by Wohlwend GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hpf+01+instrument/hpf+01+instrument/10__1128_slash_jvi__00471___12-112-12-16
Average 90 stars, based on 1 article reviews
hpf 01 instrument - by Bioz Stars, 2026-09
90/100 stars

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Infection:

Article Title: Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
Article Snippet: NIH 3T3 cells were grown on carbon-coated sapphire discs and infected with M50HA-ΔP virus at an MOI of 0.5 without or with addition of 1 μg/ml Dox for 48 h. Cells were fixed by high-pressure freezing with an HPF 01 instrument (Engineering Office, M. Wohlwend GmbH, Switzerland), freeze-substituted, and plastic embedded as described previously ( 36 ). .. NIH 3T3 cells were grown on carbon-coated sapphire discs and infected with M50HA-ΔP virus at an MOI of 0.5 without or with addition of 1 μg/ml Dox for 48 h. Cells were fixed by high-pressure freezing with an HPF 01 instrument (Engineering Office, M. Wohlwend GmbH, Switzerland), freeze-substituted, and plastic embedded as described previously ( 36 ). .. BALB/c murine embryonic fibroblasts (MEFs), human foreskin fibroblasts (HFFs), M2-10B4 bone marrow stromal cells (ATCC CRL-1972), mouse lymphoid endothelial cells immortalized by simian virus 40 (SVEC 4-10 endothelial cells; ATCC CRL-2181), and NIH 3T3 fibroblasts (ATCC CRL-1658) were prepared and treated as described previously ( 1 , 20 ).

Article Title: Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
Article Snippet: .. NIH 3T3 cells were grown on carboncoated sapphire discs and infected with M50HA- P virus at an MOI of 0.5 without or with addition of 1 g/ml Dox for 48 h. Cells were fixed by highpressure freezing with an HPF 01 instrument (Engineering Office, M. Wohlwend GmbH, Switzerland), freeze-substituted, and plastic embedded as described previously (36). ..

Article Title: A Beta-Herpesvirus with Fluorescent Capsids to Study Transport in Living Cells
Article Snippet: .. NIH 3T3 or M2-10B4 cells were grown on carbon-coated sapphire discs and infected at a MOI of 0.5 with centrifugal enhancement at 1600 g/RT for 30 min. After an additional hour, the inoculum was replaced by normal medium and cells were incubated for 48 h. Then, cells were fixed by high-pressure freezing with an HPF 01 instrument (M. Wohlwend GmbH), freeze-substituted, and plastic embedded as described previously . .. Embedded samples were thin-sectioned and viewed on a Zeiss EM 10 at 80 kV in transmission mode.

Virus:

Article Title: Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
Article Snippet: NIH 3T3 cells were grown on carbon-coated sapphire discs and infected with M50HA-ΔP virus at an MOI of 0.5 without or with addition of 1 μg/ml Dox for 48 h. Cells were fixed by high-pressure freezing with an HPF 01 instrument (Engineering Office, M. Wohlwend GmbH, Switzerland), freeze-substituted, and plastic embedded as described previously ( 36 ). .. NIH 3T3 cells were grown on carbon-coated sapphire discs and infected with M50HA-ΔP virus at an MOI of 0.5 without or with addition of 1 μg/ml Dox for 48 h. Cells were fixed by high-pressure freezing with an HPF 01 instrument (Engineering Office, M. Wohlwend GmbH, Switzerland), freeze-substituted, and plastic embedded as described previously ( 36 ). .. BALB/c murine embryonic fibroblasts (MEFs), human foreskin fibroblasts (HFFs), M2-10B4 bone marrow stromal cells (ATCC CRL-1972), mouse lymphoid endothelial cells immortalized by simian virus 40 (SVEC 4-10 endothelial cells; ATCC CRL-2181), and NIH 3T3 fibroblasts (ATCC CRL-1658) were prepared and treated as described previously ( 1 , 20 ).

Article Title: Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
Article Snippet: .. NIH 3T3 cells were grown on carboncoated sapphire discs and infected with M50HA- P virus at an MOI of 0.5 without or with addition of 1 g/ml Dox for 48 h. Cells were fixed by highpressure freezing with an HPF 01 instrument (Engineering Office, M. Wohlwend GmbH, Switzerland), freeze-substituted, and plastic embedded as described previously (36). ..

Incubation:

Article Title: A Beta-Herpesvirus with Fluorescent Capsids to Study Transport in Living Cells
Article Snippet: .. NIH 3T3 or M2-10B4 cells were grown on carbon-coated sapphire discs and infected at a MOI of 0.5 with centrifugal enhancement at 1600 g/RT for 30 min. After an additional hour, the inoculum was replaced by normal medium and cells were incubated for 48 h. Then, cells were fixed by high-pressure freezing with an HPF 01 instrument (M. Wohlwend GmbH), freeze-substituted, and plastic embedded as described previously . .. Embedded samples were thin-sectioned and viewed on a Zeiss EM 10 at 80 kV in transmission mode.



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